Two 600 tonne substations for the project, eight kilometres off the East Yorkshire coast in the North Sea, will be delivered to the port in November.
The substations, which will be at the port for six to 12 months, will be delivered by barge and transported by trailers to their temporary base.
The Port of Sunderland, which is owned by the city’s council, carried out works to make the most use of the port’s natural assets, freeing up additional land.
Councillor Paul Watson, leader of Sunderland City Council and the port board’s chair, said: "Reconfiguration work has come a long way at the port and the line-up of developments that have taken place to enhance our offer to customers, have no doubt helped to position it as an appealing base for many companies."
In May, E.on contracted Gardline to undertake construction phase environmental surveys at Humber Gateway. It is due for completion in 2015.
Matthew Hunt, port director, said: "The E.on contract is an exciting opportunity for Port of Sunderland, with a very visible workscope which allows us to demonstrate our ultra-large cargo handling capability."